Liposome can be produced in the form of multi-compartment with vesicles sizes ranging from 100 nm to 1000 nm and each vesicle usually composed of five or more concentric lamellae. In the process of its formation, the resulting liposomes with various sizes which will be the basis of classification:

a. Unilamellar Small vesicles (SUV)
SUV has the smallest size variation. Size SUV based on the strength of ionization liquid medium and fat composition of frontier membrane vesicle size ± 15 nm for the liposomes derived from pure egg lecithin in normal saline and ± 25 nm for DPPC liposome

b. Large lamellar vesicles (LUV)
Is a vesicle which has a size larger than an SUV. These liposomes have a size of 500-1000nm. Vesicles of this type can be prepared by ether injection method and the fusion of liposomes induced type SUV with calcium.

c. Intermediate-sized Unilamellar vesicles (IUV)
This liposome size of 100 nm. Usually these vesicles measuring 100-200 nm and can be made with high-pressure extrusion / detergent dialysis. These vesicles may persist longer in circulation and good stability so it is very useful in the pharmaceutical field as a drug carrier.

Size affects the distribution of liposomes and liposome blood clearance after administration systematically. Diameter or the size of the liposomes is determined by several things, among others:
  1. Lipids and combinations thereof. For example, liposomes pliers are made from a mixture of EPC and cholesterol, larger diameter (± 100-200 nm) compared with liposomes of EPC alone (<100 nm).
  2. The balance between the energy to open the liposome membrane, liposome curvature elasticity, and the amount of scattered energy.
  3. Method of making liposomes